Fillies' Mile winner Quadrilateral is set to face 14 rivals in Sunday's Qipco 1,000 Guineas at Newmarket.
The daughter of Frankel impressed during an unbeaten two-year-old campaign and has been declared to wear a tongue-strap for the first time in the Classic.
She will again face Love, who finished third in the Fillies' Mile on good to soft ground and is Aidan O'Brien's only representative in the Classic. O'Brien, who has trained five 1,000 Guineas winners including three of the last four, did not declare Peaceful and So Wonderful at the final stage, the only two runners not to stay in the race.
Like Quadrilateral and Love, Millisle was a Group 1 winner as a two-year-old and the Cheveley Park Stakes heroine will be ridden by champion jockey Oisin Murphy for the first time.
Raffle Prize winning the Queen Mary, photo Liesl King
Frankie Dettori has been confirmed to ride Shimmering for trainer John Gosden, with Mickael Barzalona drafted in on Queen Mary winner Raffle Prize for the first time.
Ladbrokes have reported that Love is their worst result for Sunday's Classic having been 20-1 at the start of May. O'Brien's only representative is now a 4-1 chance.
Nicola McGeady of Ladbrokes said: "We are certainly running scared of Love ahead of the 1,000 Guineas. She has been the momentum horse with her odds shortening over the last number of weeks. As Aidan O'Brien's only runner in the race, we imagine support for her will continue until the off."
Qipco 1,000 Guineas runners and riders
1 Boomer (Richard Kingscote), drawn 7
2 Cloak Of Spirits (Andrea Atzeni), drawn 5
3 Final Song (Pat Cosgrave), drawn 2
4 Graceful Magic (Charles Bishop), drawn 9
5 Les Hogues (Cieren Fallon), drawn 3
6 Love (Ryan Moore), drawn 14
7 Millisle (Oisin Murphy), drawn 11
8 Quadrilateral (Jason Watson), drawn 6
9 Raffle Prize (Mickael Barzalona), drawn 13
10 Romsey (Tom Marquand), drawn 8
11 Rose Of Kildare (Joe Fanning), drawn 10
12 Shimmering (Frankie Dettori), drawn 1
13 Summer Romance (William Buick), drawn 4
14 Under The Stars (PJ McDonald), drawn 12
15 Yes Always (Ben Curtis), drawn 15